Molecular Characterization of Ampicillin Resistant Poultry Isolates of Salmonella Typhimurium

Multiple drug resistance and high genetic variation among the field isolates are one of the two major constrains in eradication and control of Salmonella Typhimurium from poultry. In the present study 51 isolates of Salmonella typhimurium which were isolated from eggs were analyzed for their responses against commonly used sixteen antibiotics. Genetic variation among these isolates was studied by using ERIC-PCR, REP-PCR and AP-PCR. Most of the isolates were possessing resistance for more than three antibiotics. Maximum resistance was exhibited for Ampicillin and Augmentin. Among three (ERIC-PCR, REP-PCR and AP-PCR), AP-PCR shown highest discrimination ability D value 0.89 . On combined molecular-typing discriminative index (D value 0.984) was achieved. Present findings indicates that these isolates having high genetic variation and multiple drug resistance which may cause serious health problems in countries like India where hygienic conditions are not very good.